Ultrafine Platinum Nanoparticles Supported on Covalent Organic Frameworks As Stable and Reusable Oxidase-Like Catalysts for Cellular Glutathione Detection

Nanozymes have been widely developed as supplements of natural enzymes owing to their relatively low cost, high mass production, easy storage, good stability, and reusability. By controlling the particle size, nanozymes’ catalytic activities can distinctly be improved. The porous materials such covalent organic frameworks (COFs) are definitely considered promising supports for in situ preparation dispersity small-size nanozymes. Herein, we designed synthesized COF supported ultrafine platinum nanoparticles (Pt NPs/COF-300-AR) by growth Pt NPs using uniformly distributed nitrogen atoms well-organized framework structures 3D COFs. NPs/COF-300-AR nanohybrids catalyzed colorless 3,3?,5,5?-tetramethylbenzidine (TMB) solution turn blue, displaying excellent oxidase-like activity, reusability, which should owed uniform size NPs. virtue those advantages, TMB-Pt colorimetric platform was successfully applied cellular glutathione detection. Similarly, other COFs with acid resistance abundant functional groups also used a nanozyme, will greatly expand members nanoenzyme family. KEYWORDS: oxidase mimic, preparation, nanoparticles, support, glutathione, nanohybrids, colorimetry, cell lysate